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F12 ELISA Kit

Human F12 ELISA Kit, Colorimetric assay for quantification of Human F12.
Catalog No. ABIN8077036
$772.15
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for F12 ELISA Kit (ABIN8077036)

Target

See all F12 ELISA Kits
F12 (Coagulation Factor XII (F12))

Reactivity

  • 15
  • 13
  • 9
  • 6
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

1.56 ng/mL - 100 ng/mL

Application

ELISA

Sample Type

Plasma
  • Minimum Detection Limit

    1.56 ng/mL

    Purpose

    Human Coagulation Factor XII (F12) ELISA Kit for detecting F12 in plasma.

    Analytical Method

    Quantitative

    Sensitivity

    0.55 ng/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all F12 ELISA Kits

    F12 (Coagulation Factor XII (F12))

    Alternative Name

    Coagulation Factor XII

    Background

    Gene Aliases: FXII, HAF, Contact Factor, Hageman Factor

    Gene ID

    2161

    UniProt

    P00748

    Pathways

    Complement System
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