C3 CLIA Kit
Quick Overview for C3 CLIA Kit (ABIN8077316)
Target
See all C3 CLIA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.27 ng/mL
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Purpose
- Rat Complement Component 3 (C3) CLIA Kit for detecting C3 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 0.12 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Complement Component 3 (C3) ELISA Kit
C3 Reactivity: Pig Colorimetric Competition ELISA 0.625 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 Reactivity: Human Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 Reactivity: Rat Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 Reactivity: Mouse Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 Reactivity: Cow Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 Reactivity: Pig Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 Reactivity: Mouse Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Component 3 (C3) ELISA KitC3 Reactivity: Rat Colorimetric Competition ELISA 0.625 μg/mL - 40 μg/mL Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 Reactivity: Dog Colorimetric Competition ELISA 0.78 μg/mL - 50 μg/mL Plasma, Serum, Tissue Homogenate
Complement Component 3 (C3) ELISA KitC3 Reactivity: Rabbit Colorimetric Competition ELISA 27.3 ng/mL - 7000 ng/mL Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- C3 (Complement Component 3 (C3))
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Alternative Name
- Complement Component 3
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Background
- Gene Aliases: ASP, CPAMD1, Complement Protein C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, Acylation stimulating protein
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Gene ID
- 24232
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UniProt
- P01026
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Pathways
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
Target See all C3 CLIA Kits
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