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CRH ELISA Kit

The Mouse CRH ELISA Kit (ABIN8077542) is a Colorimetric ELISA Kit designed to quantify Mouse CRH.
Catalog No. ABIN8077542
$789.58
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for CRH ELISA Kit (ABIN8077542)

Target

See all CRH ELISA Kits
CRH (Corticotropin Releasing Hormone (CRH))

Reactivity

  • 21
  • 17
  • 17
  • 11
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
Mouse

Detection Method

Colorimetric

Method Type

Competition ELISA

Detection Range

12.35 pg/mL - 1000 pg/mL

Application

ELISA

Sample Type

Plasma, Serum
  • Minimum Detection Limit

    12.35 pg/mL

    Purpose

    Mouse Corticotropin Releasing Factor (CRF) ELISA Kit for detecting CRF in serum, plasma

    Analytical Method

    Quantitative

    Sensitivity

    5.05 pg/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    50 μL

    Assay Time

    2 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all CRH ELISA Kits

    CRH (Corticotropin Releasing Hormone (CRH))

    Alternative Name

    Corticotropin Releasing Factor

    Background

    Gene Aliases: CRH, Corticotropin Releasing Hormone, Corticoliberin

    Gene ID

    12918

    UniProt

    Q8CIT0

    Pathways

    Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour
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