Cyclic GMP ELISA Kit

Catalog No. ABIN8077699

Various Species Cyclic GMP ELISA Kit, Colorimetric assay for quantification of Various Species Cyclic GMP.

Quick Overview for Cyclic GMP ELISA Kit (ABIN8077699)

Target: Cyclic GMP (cGMP)

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 123.5 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details Cyclic GMP ELISA Kit

Minimum Detection Limit: 123.5 pg/mL

Purpose: Cyclic Guanosine Monophosphate (cGMP) ELISA Kit for detecting cGMP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensitivity: 49.4 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 50 μL

Assay Time: 2 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for Cyclic GMP

Target: Cyclic GMP (cGMP)

Alternative Name: Cyclic Guanosine Monophosphate

Target Type: Chemical

Background: Gene Aliases: c-GMP, 3',5'-Cyclic GMP, Cyclic 3',5'-GMP, Guanosine 3',5'-Cyclic Phosphate