DDIT3 ELISA Kit
Quick Overview for DDIT3 ELISA Kit (ABIN8078233)
Target
See all DDIT3 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
-
-
Purpose
- GADD153 Colorimetric Cell-Based ELISA for detecting GADD153 in researchers plate their cell line of choice.
-
Analytical Method
- Qualitative
-
Components
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
Material not included
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA Kit
DDIT3 Reactivity: Rat Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA KitDDIT3 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA KitDDIT3 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA KitDDIT3 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA KitDDIT3 Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA KitDDIT3 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA KitDDIT3 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
DNA-Damage-Inducible Transcript 3 (DDIT3) ELISA KitDDIT3 Reactivity: Rat Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Comment
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
Assay Time
- 4 - 5 h
-
Plate
- Pre-coated
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- 4°C for one month, Long term: see manual.
-
Expiry Date
- 6 months
-
-
- DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))
-
Alternative Name
- GADD153
-
Background
- Gene Aliases: C/EBP-homologous protein, DDIT3, DNA-damage inducible transcript 3, GADD153, Growth arrest and DNA-damage-inducible protein GADD153
-
Gene ID
- 1649
-
UniProt
- P35638
-
Pathways
- Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis
Target See all DDIT3 ELISA Kits
-
