EGLN1 ELISA Kit

Catalog No. ABIN8078371

Human EGLN1 ELISA Kit, Colorimetric assay for quantification of Human EGLN1.

Quick Overview for EGLN1 ELISA Kit (ABIN8078371)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details EGLN1 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Human Egl Nine Homolog 1 (EGLN1) ELISA Kit for detecting EGLN1 in serum, plasma, tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensitivity: 12.2 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: Egl Nine Homolog 1

Background: Gene Aliases: ECYT3, HIFPH2, PHD2, C1orf12, SM20, ZMYND6, HIF Prolyl Hydroxylase 2, Prolyl hydroxylase domain-containing protein 2, Hypoxia-inducible factor prolyl hydroxylase 2

Gene ID: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect