ECF ELISA Kit
Quick Overview for ECF ELISA Kit (ABIN8078534)
Target
See all ECF ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
-
-
Minimum Detection Limit
- 62.5 pg/mL
-
Purpose
- Bovine Eosinophil Chemotactic Factor (ECF) ELISA Kit for detecting ECF in serum, plasma
-
Analytical Method
- Quantitative
-
Sensitivity
- 21.8 pg/mL
-
Components
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
Material not included
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
Eosinophil Chemotactic Factor (ECF) ELISA Kit
ECF Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Eosinophil Chemotactic Factor (ECF) ELISA KitECF Reactivity: Monkey Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Eosinophil Chemotactic Factor (ECF) ELISA KitECF Reactivity: Rat Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Eosinophil Chemotactic Factor (ECF) ELISA KitECF Reactivity: Cow Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Eosinophil Chemotactic Factor (ECF) ELISA KitECF Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Eosinophil Chemotactic Factor (ECF) ELISA KitECF Reactivity: Guinea Pig Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Eosinophil Chemotactic Factor (ECF) ELISA KitECF Reactivity: Horse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Eosinophil Chemotactic Factor (ECF) ELISA KitECF Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Eosinophil Chemotactic Factor (ECF) ELISA KitECF Reactivity: Rat Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Comment
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
Sample Volume
- 100 μL
-
Assay Time
- 3 h
-
Plate
- Pre-coated
-
Assay Precision
-
Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- 4°C for one month, Long term: see manual.
-
Expiry Date
- 12 months
-
-
- ECF (Eosinophil Chemotactic Factor (ECF))
-
Alternative Name
- Eosinophil Chemotactic Factor
-
Background
- Gene Aliases: CCL11, SCYA11, Eotaxin 1, Eosinophil Chemotactic Protein 1, Chemokine C-C-Motif Ligand 11, Small Inducible Cytokine Subfamily A (Cys-Cys) Member 11
-
Gene ID
- 404072
-
UniProt
- Q9TTS6
Target See all ECF ELISA Kits
-
