G Protein Coupled Receptor 131 ELISA Kit

Catalog No. ABIN8079525

The Rat G Protein Coupled Receptor 131 ELISA Kit (ABIN8079525) is a Colorimetric ELISA Kit designed to quantify Rat G Protein Coupled Receptor 131.

Quick Overview for G Protein Coupled Receptor 131 ELISA Kit (ABIN8079525)

Target: G Protein Coupled Receptor 131

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details

Minimum Detection Limit: 0.625 ng/mL

Purpose: Rat G Protein Coupled Receptor 131 (GPR131) Mini sample ELISA Kit for detecting GPR131 in tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensitivity: 0.231 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 15 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details

Target: G Protein Coupled Receptor 131

Background: Gene Aliases: GPBAR1, GPCR, BG37, GPCR19, M-BAR, TGR5, G Protein-Coupled Bile Acid Receptor 1, G-protein coupled receptor GPCR19, Membrane-type receptor for bile acids

Gene ID: 338443

UniProt: Q80T02