GZMB ELISA Kit
Quick Overview for GZMB ELISA Kit (ABIN8080126)
Target
See all GZMB ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
-
-
Purpose
- Granzyme B Colorimetric Cell-Based ELISA Kit for detecting Granzyme B in researchers plate their cell line of choice.
-
Analytical Method
- Qualitative
-
Components
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
Material not included
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
Granzyme B (GZMB) ELISA Kit
GZMB Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granzyme B (GZMB) ELISA KitGZMB Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granzyme B (GZMB) ELISA KitGZMB Reactivity: Mouse Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Granzyme B (GZMB) ELISA KitGZMB Reactivity: Mouse Colorimetric Sandwich ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Granzyme B (GZMB) ELISA KitGZMB Reactivity: Rat Colorimetric Sandwich ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Granzyme B (GZMB) ELISA KitGZMB Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Granzyme B (GZMB) ELISA KitGZMB Reactivity: Human AA 21-247 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Comment
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
Assay Time
- 4 - 5 h
-
Plate
- Pre-coated
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- 4°C for one month, Long term: see manual.
-
Expiry Date
- 6 months
-
-
- GZMB (Granzyme B (GZMB))
-
Alternative Name
- Granzyme B
-
Background
- Gene Aliases: Granzyme B, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11
-
Gene ID
- 3002
-
UniProt
- P10144
-
Pathways
- Apoptosis, Caspase Cascade in Apoptosis
Target See all GZMB ELISA Kits
-
