Histone H2B ELISA Kit

Catalog No. ABIN8080712

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse, Human and Rat Histone H2B.

Quick Overview for Histone H2B ELISA Kit (ABIN8080712)

Target: Histone H2B

Reactivity: Mouse, Human, Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details Histone H2B ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Purpose: Human/Mouse/Rat Histone H2B (H2B) ELISA Kit for detecting H2B in tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensitivity: 1.13 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for Histone H2B

Target: Histone H2B

Background: Gene Aliases:

Gene ID: 8349

UniProt: Q16778