HSD11B2 ELISA Kit

Catalog No. ABIN8080825

Rat HSD11B2 ELISA Kit, Colorimetric assay for quantification of Rat HSD11B2.

Quick Overview for HSD11B2 ELISA Kit (ABIN8080825)

Target: HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Product Details HSD11B2 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Purpose: Rat 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) ELISA Kit for detecting HSD11b2 in tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensitivity: 0.31 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for HSD11B2

Target: HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))

Alternative Name: 11-Beta-Hydroxysteroid Dehydrogenase Type 2

Background: Gene Aliases: AME, AME1, HSD11K, HSD2, SDR9C3, Short Chain Dehydrogenase/Reductase Family 9C,Member 3, NAD-dependent 11-beta-hydroxysteroid dehydrogenase

Gene ID: 25117

UniProt: P50233

Pathways: Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones