HSD17B3 ELISA Kit
This Colorimetric ELISA kit is designed for the quantitative measurement of Rat HSD17B3.
Catalog No. ABIN8080832
$898.15
Plus shipping costs $50.00
96 tests
Shipping to:
United States
Delivery in 8 to 13 Business Days
Quick Overview for HSD17B3 ELISA Kit (ABIN8080832)
Target
See all HSD17B3 ELISA Kits
HSD17B3
(Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))
Reactivity
Rat
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.312 ng/mL - 20 ng/mL
Application
ELISA
Sample Type
Cell Lysate, Tissue Homogenate
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Minimum Detection Limit
- 0.312 ng/mL
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Purpose
- Rat 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) ELISA Kit for detecting HSD17b3 in tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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Sensitivity
- 0.117 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3) ELISA Kit
HSD17B3 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- HSD17B3 (Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))
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Alternative Name
- 17-Beta-Hydroxysteroid Dehydrogenase Type 3
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Background
- Gene Aliases: SDR12C2, EDH17B3, Short Chain Dehydrogenase/Reductase Family 12C,Member 2, Testosterone 17-Beta-Dehydrogenase 3, Testicular 17-beta-hydroxysteroid dehydrogenase
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Gene ID
- 117182
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UniProt
- O54939
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Pathways
- Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis
Target See all HSD17B3 ELISA Kits
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