IgM ELISA Kit
Quick Overview for IgM ELISA Kit (ABIN8081005)
Target
See all IgM ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 1.56 ng/mL
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Purpose
- Rabbit Immunoglobulin M (IgM) ELISA Kit for detecting IgM in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 0.69 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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IgM ELISA Kit
Reactivity: Chicken Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
IgM ELISA KitReactivity: Human Colorimetric Sandwich ELISA 31.2 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Grouper (Epinephelinae) Colorimetric Competition ELISA 1.25-50 μg/mL Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Cow Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Goat Colorimetric Sandwich ELISA 0.234 ng/mL - 15 ng/mL Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Rabbit Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Sheep Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Cow Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgM ELISA KitReactivity: Horse Colorimetric Sandwich ELISA 10 ng/mL - 320 ng/mL Plasma, Serum, Urine
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- IgM
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Alternative Name
- Immunoglobulin M
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Target Type
- Antibody
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Background
- Gene Aliases: IGHM, Immunoglobulin Heavy Constant Mu, Ig mu chain C region
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UniProt
- A0A5F9
Target See all IgM ELISA Kits
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