Integrin alpha 1 ELISA Kit

Catalog No. ABIN8081293

Mouse Integrin alpha 1 ELISA Kit, Colorimetric assay for quantification of Mouse Integrin alpha 1.

Quick Overview for Integrin alpha 1 ELISA Kit (ABIN8081293)

Target: Integrin alpha 1 (ITGA1)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details Integrin alpha 1 ELISA Kit

Minimum Detection Limit: 78.12 pg/mL

Purpose: Mouse Integrin Alpha 1 (ITGa1) ELISA Kit for detecting ITGa1 in tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensitivity: 31 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for Integrin alpha 1

Target: Integrin alpha 1 (ITGA1)

Alternative Name: Integrin Alpha 1

Background: Gene Aliases: CD49-A, CD49a, ITG-A1, VLA1, CD49 antigen-like family member A, Laminin and collagen receptor

Gene ID: 109700

UniProt: Q3V3R4

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex