Iba1 ELISA Kit

Catalog No. ABIN8082350

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat Iba1.

Quick Overview for Iba1 ELISA Kit (ABIN8082350)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Iba1 ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Purpose: Rat AIF1(Allograft Inflammatory Factor 1) ELISA Kit for detecting AIF1 in Serum, Plasma

Analytical Method: Quantitative

Sensitivity: 18.75 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 2-8°C for 12 months, Long term: see manual.

Expiry Date: 12 months

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: Gene Aliases: AIF1, AIF-1, IBA1, IRT-1, IRT1, allograft inflammatory factor 1

UniProt: P55009

Pathways: Smooth Muscle Cell Migration