Cytokeratin 18 ELISA Kit
Quick Overview for Cytokeratin 18 ELISA Kit (ABIN8082496)
Target
See all Cytokeratin 18 (KRT18) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.156 ng/mL
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Purpose
- Rat Cytokeratin 18 (CK18) ELISA Kit for detecting CK18 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 0.056 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Keratin 18 (KRT18) ELISA Kit
KRT18 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Keratin 18 (KRT18) ELISA KitKRT18 Reactivity: Human Colorimetric Sandwich ELISA 6.25 mIU/mL - 400 mIU/mL Cell Culture Supernatant, Plasma, Serum
Keratin 18 (KRT18) ELISA KitKRT18 Reactivity: Human Colorimetric Sandwich ELISA 15.6 mIU/mL - 1000 mIU/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Keratin 18 (KRT18) ELISA KitKRT18 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 μIU/mL - 1000 μIU/mL Plasma, Serum, Tissue Homogenate
Keratin 18 (KRT18) ELISA KitKRT18 Reactivity: Rat Colorimetric Sandwich ELISA 1.56 mIU/mL - 100 mIU/mL Plasma, Serum, Tissue Homogenate
Keratin 18 (KRT18) ELISA KitKRT18 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Keratin 18 (KRT18) ELISA KitKRT18 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Keratin 18 (KRT18) ELISA KitKRT18 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Keratin 18 (KRT18) ELISA KitKRT18 Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Keratin 18 (KRT18) ELISA KitKRT18 Reactivity: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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Alternative Name
- Cytokeratin 18
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Background
- Gene Aliases: KRT18, CYK18, K18, Keratin 18, Cell proliferation-inducing gene 46 protein, Keratin, type I cytoskeletal 18
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Gene ID
- 294853
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UniProt
- Q5BJY9
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Pathways
- Apoptosis, Caspase Cascade in Apoptosis
Target See all Cytokeratin 18 (KRT18) ELISA Kits
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