LIPE ELISA Kit

Catalog No. ABIN8082910

Mouse, Human and Rat LIPE ELISA Kit, Colorimetric assay for quantification of Mouse, Human and Rat LIPE.

Quick Overview for LIPE ELISA Kit (ABIN8082910)

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Binding Specificity: pSer552

Reactivity: Mouse, Human, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details LIPE ELISA Kit

Purpose: Phospho-HSL (Ser552) Colorimetric Cell-Based ELISA Kit for detecting Phospho-HSL (Ser552) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for LIPE

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Alternative Name: HSL

Background: Gene Aliases: Hormone sensitive lipase, LIPE, LIPS

Gene ID: 3991

UniProt: Q05469

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism