MIF ELISA Kit

Catalog No. ABIN8083131

The Rat MIF ELISA Kit (ABIN8083131) is a Colorimetric ELISA Kit designed to quantify Rat MIF.

Quick Overview for MIF ELISA Kit (ABIN8083131)

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details MIF ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Purpose: Rat MIF (Macrophage Migration Inhibitory Factor) ELISA Kit for detecting MIF in Serum, Plasma

Analytical Method: Quantitative

Sensitivity: 0.94 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 2-8°C for 12 months, Long term: see manual.

Expiry Date: 12 months

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF

Background: Gene Aliases: MIF, GIF, GLIF, Mmacrophage migration inhibitory factor (glycosylation-inhibiting factor) , Macrophage migration inhibitory factor

UniProt: P30904

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling