MADD ELISA Kit

Catalog No. ABIN8083198

Human, Mouse and Rat MADD ELISA Kit, Colorimetric assay for quantification of Human, Mouse and Rat MADD.

Quick Overview for MADD ELISA Kit (ABIN8083198)

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details MADD ELISA Kit

Purpose: MADD Colorimetric Cell-Based ELISA for detecting MADD in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for MADD

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Alternative Name: MADD

Background: Gene Aliases: Differentially expressed in normal and neoplastic cells, Insulinoma glucagonoma clone 20, Rab3 GDP/GTP exchange factor

Gene ID: 8567

UniProt: Q8WXG6

Pathways: Caspase Cascade in Apoptosis