MED1 ELISA Kit

Catalog No. ABIN8083425

The Human and Mouse MED1 ELISA Kit (ABIN8083425) is a Colorimetric ELISA Kit designed to quantify Human and Mouse MED1.

Quick Overview for MED1 ELISA Kit (ABIN8083425)

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Reactivity: Human, Mouse

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details MED1 ELISA Kit

Purpose: MED1 Colorimetric Cell-Based ELISA for detecting MED1 in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Alternative Name: MED1

Background: Gene Aliases: Mediator complex subunit 1, Peroxisome proliferator-activated receptor-binding protein, PBP, PPAR-binding protein, Thyroid hormone receptor-associated protein complex 220 kDa component, Trap220, Thyroid receptor-interacting protein 2, TRIP-2, p53 regulatory protein RB18A, Vitamin D receptor-interacting protein complex component DRIP205, Activator-recruited cofactor 205 kDa component, ARC205

Gene ID: 5469

UniProt: Q15648

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha