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MTHFR ELISA Kit

Human MTHFR ELISA Kit, Colorimetric assay for quantification of Human MTHFR.
Catalog No. ABIN8083546
$840.00
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for MTHFR ELISA Kit (ABIN8083546)

Target

See all MTHFR ELISA Kits
MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))

Reactivity

  • 9
  • 2
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

62.5 pg/mL - 4000 pg/mL

Application

ELISA

Sample Type

Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    62.5 pg/mL

    Purpose

    Human Methylenetetrahydrofolate Reductase (MTHFR) ELISA Kit for detecting MTHFR in serum, plasma, tissue homogenates

    Analytical Method

    Quantitative

    Sensitivity

    23.5 pg/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all MTHFR ELISA Kits

    MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))

    Alternative Name

    Methylenetetrahydrofolate Reductase

    Background

    Gene Aliases: 5,10-Methylenetetrahydrofolate Reductase (NADPH)

    Gene ID

    4524

    UniProt

    P42898

    Pathways

    Methionine Biosynthetic Process
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