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MAP1LC3A ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat MAP1LC3A.
Catalog No. ABIN8083601
$971.77
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for MAP1LC3A ELISA Kit (ABIN8083601)

Target

See all MAP1LC3A ELISA Kits
MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity

  • 11
  • 4
  • 3
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.312 ng/mL - 20 ng/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
  • Minimum Detection Limit

    0.312 ng/mL

    Purpose

    Rat Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) ELISA Kit for detecting MAP1LC3a in tissue homogenates, cell lysates, cell culture supernates

    Analytical Method

    Quantitative

    Sensitivity

    0.122 ng/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all MAP1LC3A ELISA Kits

    MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

    Alternative Name

    Microtubule Associated Protein 1 Light Chain 3 Alpha

    Background

    Gene Aliases: MAP1ALC3, MAP1BLC3, LC3, LC3A, ATG8E, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1

    Gene ID

    362245

    UniProt

    Q6XVN8

    Pathways

    Autophagy
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