LC3B ELISA Kit

Catalog No. ABIN8083602

The Mouse LC3B ELISA Kit (ABIN8083602) is a Colorimetric ELISA Kit designed to quantify Mouse LC3B.

Quick Overview for LC3B ELISA Kit (ABIN8083602)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Product Details LC3B ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: Mouse Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b) ELISA Kit for detecting MAP1LC3b in tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensitivity: 0.054 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: Microtubule Associated Protein 1 Light Chain 3 Beta

Background: Gene Aliases: MAP1A/1BLC3, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2

Gene ID: 67443

UniProt: Q9CQV6

Pathways: Autophagy