Erk MAP Kinase ELISA Kit

Catalog No. ABIN8083699

Human Erk MAP Kinase ELISA Kit, Colorimetric assay for quantification of Human Erk MAP Kinase.

Quick Overview for Erk MAP Kinase ELISA Kit (ABIN8083699)

Target: Erk MAP Kinase (RL) (Mitogen-Activated Protein Kinase ERK-A (RL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Erk MAP Kinase ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Purpose: Human pERK1/2 (Phospho Extracellular Signal Regulated Kinase 1/2) ELISA Kit for detecting pERK1, 2 in serum, plasma

Analytical Method: Quantitative

Sensitivity: 18.75 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 2-8°C for 12 months, Long term: see manual.

Expiry Date: 12 months

Target Details for Erk MAP Kinase

Target: Erk MAP Kinase (RL) (Mitogen-Activated Protein Kinase ERK-A (RL))

Alternative Name: pERK1, 2

Background: Gene Aliases:

UniProt: P28482