ASAH2 ELISA Kit
Quick Overview for ASAH2 ELISA Kit (ABIN8084049)
Target
See all ASAH2 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.156 ng/mL
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Purpose
- Rat N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit for detecting ASAH2 in tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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Sensitivity
- 0.055 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) ELISA Kit
ASAH2 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) ELISA KitASAH2 Reactivity: Human AA 99-780 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) ELISA KitASAH2 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) ELISA KitHigh Sensitivity ASAH2 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))
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Alternative Name
- N-Acylsphingosine Amidohydrolase 2
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Background
- Gene Aliases: HNAC1, non-lysosomal ceramidase, Neutral ceramidase
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Gene ID
- 114104
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UniProt
- Q91XT9
Target See all ASAH2 ELISA Kits
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