NQO1 ELISA Kit
Quick Overview for NQO1 ELISA Kit (ABIN8084059)
Target
See all NQO1 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.312 ng/mL
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Purpose
- Human NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit for detecting NQO1 in tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 0.124 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))
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Alternative Name
- NADH Dehydrogenase, Quinone 1
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Background
- Gene Aliases: DHQU, DIA4, DTD, NMOR1, NMORI, QR1, Azoreductase, DT-diaphorase, Menadione reductase, Phylloquinone reductase, Quinone reductase 1, NAD (P) H:quinone oxidoreductase 1
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Gene ID
- 1728
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UniProt
- P15559
Target See all NQO1 ELISA Kits
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