Nerve Growth Factor ELISA Kit
Quick Overview for Nerve Growth Factor ELISA Kit (ABIN8084149)
Target
See all Nerve Growth Factor (NGF) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.156 ng/mL
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Purpose
- Bovine Nerve Growth Factor (NGF) ELISA Kit for detecting NGF in serum, plasma, tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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Sensitivity
- 0.06 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Nerve Growth Factor (NGF) ELISA Kit
NGF Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Guinea Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitHigh Sensitivity NGF Reactivity: Human Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Rat AA 122-241 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Serum
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Chicken Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor (NGF) ELISA KitNGF Reactivity: Pig Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- Nerve Growth Factor (NGF)
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Alternative Name
- Nerve Growth Factor
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Background
- Gene Aliases: NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor
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Gene ID
- 281350
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UniProt
- P13600
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Pathways
- Regulation of Cell Size
Target See all Nerve Growth Factor (NGF) ELISA Kits
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