ENOS ELISA Kit
Quick Overview for ENOS ELISA Kit (ABIN8084427)
Target
See all ENOS (NOS3) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 1.56 ng/mL
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Purpose
- Rat Endothelial NOS (eNOS) ELISA Kit for detecting eNOS in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 0.65 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA Kit
NOS3 Reactivity: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 Reactivity: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 Reactivity: Cow Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 Reactivity: Guinea Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 Reactivity: Rabbit Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 Reactivity: Human AA 2-1203 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant
Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) ELISA KitNOS3 Reactivity: Pig Colorimetric Sandwich ELISA 3.12 IU/mL - 200 IU/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
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Alternative Name
- Endothelial NOS
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Background
- Gene Aliases: NOS3, ECNOS, NOS III, E-NOS, C-NOS, ENOS, CNOS, Constitutive NOS, Nitric Oxide Synthase 3, Endothelial
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Gene ID
- 24600
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UniProt
- Q62600
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Pathways
- ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target See all ENOS (NOS3) ELISA Kits
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