NFkB CLIA Kit
Quick Overview for NFkB CLIA Kit (ABIN8084508)
Target
See all NFkB CLIA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 27.4 pg/mL
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Purpose
- Rat Nuclear Factor Kappa B (NFkB) CLIA Kit for detecting NFkB in tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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Sensitivity
- 12.5 pg/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Nuclear Factor kappa B (NFkB) ELISA Kit
NFkB Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB Reactivity: Rat Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB Reactivity: Rabbit Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB Reactivity: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB Reactivity: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB Reactivity: Mouse Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor kappa B (NFkB) ELISA KitNFkB Reactivity: Chicken Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- NFkB (Nuclear Factor kappa B (NFkB))
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Alternative Name
- Nuclear Factor Kappa B
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Background
- Gene Aliases: NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit
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Gene ID
- 81736
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UniProt
- Q63369
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Pathways
- Ubiquitin Proteasome Pathway, S100 Proteins
Target See all NFkB CLIA Kits
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