PPARA ELISA Kit
Quick Overview for PPARA ELISA Kit (ABIN8085059)
Target
See all PPARA ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.312 ng/mL
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Purpose
- Human Peroxisome Proliferator Activated Receptor Alpha (PPARa) ELISA Kit for detecting PPARa in tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 0.116 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA Kit
PPARA Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
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Alternative Name
- Peroxisome Proliferator Activated Receptor Alpha
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Background
- Gene Aliases: NR1C1, PPAR-A, HPPAR, Nuclear Receptor Subfamily 1 Group C Member 1
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Gene ID
- 5465
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UniProt
- Q07869
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Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C
Target See all PPARA ELISA Kits
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