PLA2G2D ELISA Kit

Catalog No. ABIN8085167

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse PLA2G2D.

Quick Overview for PLA2G2D ELISA Kit (ABIN8085167)

Target: PLA2G2D (Phospholipase A2, Group IID (PLA2G2D))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details PLA2G2D ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Mouse Phospholipase A2, Group IID (PLA2G2D) ELISA Kit for detecting PLA2G2D in serum, plasma, tissue homogenates

Analytical Method: Quantitative

Sensitivity: 11.7 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for PLA2G2D

Target: PLA2G2D (Phospholipase A2, Group IID (PLA2G2D))

Alternative Name: Phospholipase A2, Group IID

Background: Gene Aliases: PLA2G2D, sPLA2S, s-PLA2, sPLA2, SPLASH, Secreted Phospholipase A2, Phosphatidylcholine 2-acylhydrolase 2D, Secretory-type PLA, stroma-associated homolog

Gene ID: 18782

UniProt: Q9WVF6

Pathways: Inositol Metabolic Process