PLA2G4A ELISA Kit

Catalog No. ABIN8085169

Mouse PLA2G4A ELISA Kit, Colorimetric assay for quantification of Mouse PLA2G4A.

Quick Overview for PLA2G4A ELISA Kit (ABIN8085169)

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details PLA2G4A ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Mouse Cytosolic Phospholipase A2 (PLA2G4) ELISA Kit for detecting PLA2G4 in serum, plasma, tissue homogenates

Analytical Method: Quantitative

Sensitivity: 24.9 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for PLA2G4A

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Alternative Name: Cytosolic Phospholipase A2

Background: Gene Aliases: c-PLA2, cPLA2, PLA2G4A, Phospholipase A2, Cytosolic, Phospholipase A2,Group IV (Cytosolic,Calcium-Dependent) , Phosphatidylcholine 2-acylhydrolase, Lysophospholipase

Gene ID: 18783

UniProt: P47713

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling