PIM3 CLIA Kit

Catalog No. ABIN8085226

Human PIM3 ELISA Kit, Chemiluminescent assay for quantification of Human PIM3.

Quick Overview for PIM3 CLIA Kit (ABIN8085226)

Target: PIM3 (Pim-3 Oncogene (PIM3))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 27.4 pg/mL - 20000 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Product Details PIM3 CLIA Kit

Minimum Detection Limit: 27.4 pg/mL

Purpose: Human Pim-3 Oncogene (PIM3) CLIA Kit for detecting PIM3 in tissue homogenates

Analytical Method: Quantitative

Sensitivity: 9.6 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details

Target: PIM3 (Pim-3 Oncogene (PIM3))

Alternative Name: Pim-3 Oncogene

Background: Gene Aliases: Serine/threonine-protein kinase pim-3

Gene ID: 415116

UniProt: Q86V86

Pathways: Negative Regulation of Hormone Secretion