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Pituitary Adenylate Cyclase Activating Peptide ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Pituitary Adenylate Cyclase Activating Peptide.
Catalog No. ABIN8085227
$1,069.15
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for Pituitary Adenylate Cyclase Activating Peptide ELISA Kit (ABIN8085227)

Target

Pituitary Adenylate Cyclase Activating Peptide

Reactivity

  • 4
  • 2
Mouse

Detection Method

Colorimetric

Method Type

Competition ELISA

Detection Range

24.69 pg/mL - 2000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Plasma, Serum
  • Minimum Detection Limit

    24.69 pg/mL

    Purpose

    Mouse PACAP ELISA Kit for detecting Pituitary Adenylate Cyclase Activating Peptide in Serum, Plasma, Cell Culture Supernates

    Analytical Method

    Quantitative

    Sensitivity

    9.19 pg/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    50 μL

    Assay Time

    2 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target

    Pituitary Adenylate Cyclase Activating Peptide

    Background

    Gene Aliases: ADCYAP1, PRP-48, Pituitary Adenylate Cyclase Activating Peptide

    Gene ID

    11516

    UniProt

    O70176
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