P4HB ELISA Kit
Quick Overview for P4HB ELISA Kit (ABIN8085714)
Target
See all P4HB ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 3.12 pg/mL
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Purpose
- Human Protein Disulfide Isomerase (PDI) ELISA Kit for detecting PDI in serum, plasma, tissue homogenates
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Analytical Method
- Quantitative
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Sensitivity
- 1.37 pg/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) ELISA Kit
P4HB Reactivity: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) ELISA KitP4HB Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) ELISA KitP4HB Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) ELISA KitP4HB Reactivity: Human AA 18-508 Colorimetric Sandwich ELISA 93.8 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) ELISA KitP4HB Reactivity: Human Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) ELISA KitP4HB Reactivity: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) ELISA KitP4HB Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
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Alternative Name
- Protein Disulfide Isomerase
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Background
- Gene Aliases: P4HB, ERBA2L, PDIA1, PO4DB, PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta, Prolyl 4-Hydroxylase Subunit Beta, Procollagen-Proline 2-Oxoglutarate 4-Dioxygenase Beta Polypeptide
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Gene ID
- 5034
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UniProt
- P07237
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Pathways
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
Target See all P4HB ELISA Kits
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