PCCA ELISA Kit
This Colorimetric ELISA kit is designed for the quantitative measurement of Human PCCA.
Catalog No. ABIN8085734
$840.00
Plus shipping costs $50.00
96 tests
Shipping to:
United States
Delivery in 8 to 13 Business Days
Quick Overview for PCCA ELISA Kit (ABIN8085734)
Target
See all PCCA products
PCCA
(Propionyl CoA Carboxylase, alpha Polypeptide (PCCA))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.312 ng/mL - 20 ng/mL
Application
ELISA
Sample Type
Cell Lysate, Tissue Homogenate
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Minimum Detection Limit
- 0.312 ng/mL
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Purpose
- Human Propionyl Coenzyme A Carboxylase Alpha (PCCa) ELISA Kit for detecting PCCa in tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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Sensitivity
- 0.112 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Propionyl CoA Carboxylase, alpha Polypeptide (PCCA) ELISA Kit
PCCA Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- PCCA (Propionyl CoA Carboxylase, alpha Polypeptide (PCCA))
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Alternative Name
- Propionyl Coenzyme A Carboxylase Alpha
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Background
- Gene Aliases: Propanoyl-CoA:carbon dioxide ligase subunit alpha, Propionyl-CoA carboxylase alpha chain, mitochondrial
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Gene ID
- 5095
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UniProt
- P05165
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Pathways
- Monocarboxylic Acid Catabolic Process
Target See all PCCA products
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