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PDIA3 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PDIA3.
Catalog No. ABIN8085833
$806.08
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for PDIA3 ELISA Kit (ABIN8085833)

Target

See all PDIA3 ELISA Kits
PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivity

  • 10
  • 6
  • 5
  • 4
  • 3
  • 1
  • 1
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Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.156 ng/mL - 10 ng/mL

Application

ELISA

Sample Type

Cell Lysate, Tissue Homogenate
  • Minimum Detection Limit

    0.156 ng/mL

    Purpose

    Human Endoplasmic Reticulum Resident Protein 57 (ERp57) ELISA Kit for detecting ERp57 in tissue homogenates, cell lysates

    Analytical Method

    Quantitative

    Sensitivity

    0.056 ng/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all PDIA3 ELISA Kits

    PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

    Alternative Name

    Endoplasmic Reticulum Resident Protein 57

    Background

    Gene Aliases: P58, ERp61, PDIA3, ERp60, GRP57, PI-PLC, GRP58, Glucose Regulated Protein,58 kDa, Protein Disulfide Isomerase-Associated 3, Endoplasmic reticulum resident protein 57

    Gene ID

    2923

    UniProt

    P30101

    Pathways

    Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis
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