PKC alpha ELISA Kit
Quick Overview for PKC alpha ELISA Kit (ABIN8085845)
Target
See all PKC alpha (PKCa) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.312 ng/mL
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Purpose
- Rat Protein Kinase C Alpha (PKCa) ELISA Kit for detecting PKCa in tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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Sensitivity
- 0.117 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Protein Kinase C, alpha (PKCa) ELISA Kit
PKCa Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Mouse Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Rat Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Mouse Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Protein Kinase C, alpha (PKCa) ELISA KitPKCa Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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Alternative Name
- Protein Kinase C Alpha
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Background
- Gene Aliases: PRKCA, PKC-A, AAG6, PKC-Alpha
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UniProt
- P05696
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Pathways
- WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target See all PKC alpha (PKCa) ELISA Kits
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