PKC beta ELISA Kit

Catalog No. ABIN8085850

Human, Rat and Mouse PKC beta ELISA Kit, Colorimetric assay for quantification of Human, Rat and Mouse PKC beta.

Quick Overview for PKC beta ELISA Kit (ABIN8085850)

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Binding Specificity: pSer661

Reactivity: Human, Rat, Mouse

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details PKC beta ELISA Kit

Purpose: Phospho-PKCB (Ser661) Colorimetric Cell-Based ELISA Kit for detecting Phospho-PKCB (Ser661) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Alternative Name: PKCB

Background: Gene Aliases: kinase PKC-beta, KPCB, PKC II, PKC-B, PKC-beta, PKC-II, PRKCB, PRKCB1, Protein kinase C beta type

Gene ID: 5579

UniProt: P05771

Pathways: WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling