PKC epsilon ELISA Kit
Quick Overview for PKC epsilon ELISA Kit (ABIN8085865)
Target
See all PKC epsilon (PRKCE) ELISA KitsBinding Specificity
Reactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- Phospho-PKC ε (Ser729) Colorimetric Cell-Based ELISA Kit for detecting Phospho-PKC ε (Ser729) in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Protein Kinase C, epsilon (PRKCE) ELISA Kit
PRKCE Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivity: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivity: Mouse Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivity: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivity: Mouse Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Protein Kinase C, epsilon (PRKCE) ELISA KitPRKCE Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 4 - 5 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 6 months
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- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
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Alternative Name
- PKC epsilon
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Background
- Gene Aliases: KPCE, PKC-epsilon, PKCEA, PRKCE, Protein kinase C, epsilon type, kinase PKC-epsilon, nPKC-epsilon
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Gene ID
- 5581
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UniProt
- Q02156
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Pathways
- TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
Target See all PKC epsilon (PRKCE) ELISA Kits
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