PKM2 ELISA Kit

Catalog No. ABIN8086062

The Human PKM2 ELISA Kit (ABIN8086062) is a Colorimetric ELISA Kit designed to quantify Human PKM2.

Quick Overview for PKM2 ELISA Kit (ABIN8086062)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details PKM2 ELISA Kit

Minimum Detection Limit: 78 pg/mL

Purpose: Human Pyruvate kinase isozymes M2 (PKM2) ELISA Kit for detecting PKM2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensitivity: 29 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: Pyruvate kinase isozymes M2

Background: Gene Aliases: M2PK, PKM, M2-PK, PKM1, CTHBP, OIP3, PK3, PKM, TCB, THBP1, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Cytosolic thyroid hormone-binding protein, Pyruvate Kinase, Muscle

Gene ID: 5315

UniProt: P14618

Pathways: Warburg Effect