RIPK2 ELISA Kit

Catalog No. ABIN8086158

The Human and Mouse RIPK2 ELISA Kit (ABIN8086158) is a Colorimetric ELISA Kit designed to quantify Human and Mouse RIPK2.

Quick Overview for RIPK2 ELISA Kit (ABIN8086158)

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Binding Specificity: pSer176

Reactivity: Human, Mouse

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details RIPK2 ELISA Kit

Purpose: Phospho-RIPK2 (Ser176) Colorimetric Cell-Based ELISA Kit for detecting Phospho-RIPK2 (Ser176) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for RIPK2

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Alternative Name: RIPK2

Background: Gene Aliases: CARD-containing IL-1 beta ICE-kinase, CARD-containing interleukin-1 beta converting enzyme associated kinase, CARDIAK, kinase RIPK2, Receptor-interacting protein 2, Receptor-interacting serine/threonine protein kinase 2, RICK, RIP-2, RIP-like interacting CLARP kinase, RIP2, RIPK2

Gene ID: 8767

UniProt: O43353

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades