S100A8 ELISA Kit
Quick Overview for S100A8 ELISA Kit (ABIN8086521)
Target
See all S100A8 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 15.6 pg/mL
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Purpose
- Pig S100 Calcium Binding Protein A8 (S100A8) ELISA Kit for detecting S100A8 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 5.2 pg/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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S100 Calcium Binding Protein A8 (S100A8) ELISA Kit
S100A8 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 Reactivity: Human Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 Reactivity: Human Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 Reactivity: Mouse Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 Reactivity: Cow Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 Reactivity: Human AA 2-93 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 Reactivity: Mouse AA 2-89 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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Alternative Name
- S100 Calcium Binding Protein A8
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Background
- Gene Aliases: MRP8, MIF, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, NIF, P8, Calgranulin A, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8
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Gene ID
- 100127488
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UniProt
- C3S7K5
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Pathways
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
Target See all S100A8 ELISA Kits
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