SFRP1 ELISA Kit

Catalog No. ABIN8086609

Mouse SFRP1 ELISA Kit, Colorimetric assay for quantification of Mouse SFRP1.

Quick Overview for SFRP1 ELISA Kit (ABIN8086609)

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details SFRP1 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Mouse Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit for detecting SFRP1 in serum, plasma, tissue homogenates

Analytical Method: Quantitative

Sensitivity: 12.7 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for SFRP1

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Alternative Name: Secreted Frizzled Related Protein 1

Background: Gene Aliases: FRP1, FRP, FRP-1, FrzA, SARP2, Secreted apoptosis-related protein 2

Gene ID: 20377

UniProt: Q8C4U3

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation