Osteopontin ELISA Kit
Quick Overview for Osteopontin ELISA Kit (ABIN8086627)
Target
See all Osteopontin (SPP1) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 39 pg/mL
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Purpose
- Mouse Osteopontin (OPN) ELISA Kit for detecting OPN in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 15 pg/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Secreted phosphoprotein 1 (SPP1) ELISA Kit
SPP1 Reactivity: Dog Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 Reactivity: Human Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 Reactivity: Mouse Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 Reactivity: Human Colorimetric Sandwich ELISA 50 pg/mL - 18000 pg/mL Cell Culture Supernatant, Plasma, Serum
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 Reactivity: Human AA 17-300 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 Reactivity: Mouse AA 17-294 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 Reactivity: Mouse Colorimetric Sandwich ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 Reactivity: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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Alternative Name
- Osteopontin
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Background
- Gene Aliases: SPP1, BNSP, BSPI, ETA1, Secreted Phosphoprotein 1, Bone Sialoprotein I, Early T-Lymphocyte Activation 1, Nephropontin, Urinary stone protein, Uropontin
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Gene ID
- 20750
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UniProt
- P10923
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Pathways
- Regulation of Cell Size
Target See all Osteopontin (SPP1) ELISA Kits
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