Sonic Hedgehog ELISA Kit
Quick Overview for Sonic Hedgehog ELISA Kit (ABIN8087244)
Target
See all Sonic Hedgehog (SHH) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 1.56 ng/mL
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Purpose
- Rat Hedgehog Homolog, Sonic (SHH) ELISA Kit for detecting SHH in tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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Sensitivity
- 0.59 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Sonic Hedgehog (SHH) ELISA Kit
SHH Reactivity: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH Reactivity: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH Reactivity: Mouse Colorimetric Sandwich ELISA 28 pg/mL - 1800 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH Reactivity: Mouse Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSmall Sample SHH Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH Reactivity: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH Reactivity: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- Sonic Hedgehog (SHH)
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Alternative Name
- Hedgehog Homolog, Sonic
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Background
- Gene Aliases: HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N
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Gene ID
- 29499
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UniProt
- Q63673
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Pathways
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
Target See all Sonic Hedgehog (SHH) ELISA Kits
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