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SPARCL1 ELISA Kit

Human SPARCL1 ELISA Kit, Colorimetric assay for quantification of Human SPARCL1.
Catalog No. ABIN8087278
$612.23
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for SPARCL1 ELISA Kit (ABIN8087278)

Target

See all SPARCL1 ELISA Kits
SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Reactivity

  • 14
  • 10
  • 5
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.312 ng/mL - 20 ng/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    0.312 ng/mL

    Purpose

    Human SPARC Like Protein 1 (SPARCL1) ELISA Kit for detecting SPARCL1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

    Analytical Method

    Quantitative

    Sensitivity

    0.113 ng/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all SPARCL1 ELISA Kits

    SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

    Alternative Name

    SPARC Like Protein 1

    Background

    Gene Aliases: SC1, PIG33, Mast9, Hevin, High endothelial venule protein

    Gene ID

    8404

    UniProt

    Q14515
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