SPHK1 ELISA Kit

Catalog No. ABIN8087315

This Colorimetric ELISA kit is designed for the quantitative measurement of Human SPHK1.

Quick Overview for SPHK1 ELISA Kit (ABIN8087315)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details SPHK1 ELISA Kit

Minimum Detection Limit: 0.312 ng/mL

Purpose: Human Sphingosine Kinase 1 (SPHK1) ELISA Kit for detecting SPHK1 in tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensitivity: 0.117 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: Sphingosine Kinase 1

Background: Gene Aliases:

Gene ID: 8877

UniProt: Q9NYA1

Pathways: VEGF Signaling