SPON1 ELISA Kit

Catalog No. ABIN8087334

The Human SPON1 ELISA Kit (ABIN8087334) is a Colorimetric ELISA Kit designed to quantify Human SPON1.

Quick Overview for SPON1 ELISA Kit (ABIN8087334)

Target: SPON1 (Spondin 1 (SPON1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details SPON1 ELISA Kit

Minimum Detection Limit: 0.625 ng/mL

Purpose: Human Spondin 1 (SPON1) ELISA Kit for detecting SPON1 in serum, plasma

Analytical Method: Quantitative

Sensitivity: 0.211 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for SPON1

Target: SPON1 (Spondin 1 (SPON1))

Alternative Name: Spondin 1

Background: Gene Aliases: VSGP, F-spondin, F-spondin,Extracellular Matrix Protein, Vascular smooth muscle cell growth-promoting factor

Gene ID: 10418

UniProt: Q9HCB6