Surfactant Protein A1 ELISA Kit

Catalog No. ABIN8087507

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Surfactant Protein A1.

Quick Overview for Surfactant Protein A1 ELISA Kit (ABIN8087507)

Target: Surfactant Protein A1 (SFTPA1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.88 pg/mL - 3000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details Surfactant Protein A1 ELISA Kit

Minimum Detection Limit: 46.88 pg/mL

Purpose: Human Pulmonary Surfactant Associated Protein A1 (SFTPA1) ELISA Kit for detecting SFTPA1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensitivity: 16.99 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for Surfactant Protein A1

Target: Surfactant Protein A1 (SFTPA1)

Alternative Name: Pulmonary Surfactant Associated Protein A1

Background: Gene Aliases: PRL, SFTPA, PSAP, PSPA, SFTP, SPA, COLEC4, PSP-A, SFTP1, SFTPA1B, SP-A1, Collectin-4, Alveolar proteinosis protein, 35 kDa pulmonary surfactant-associated protein, Surfactant Associated Protein A

Gene ID: 653509

UniProt: Q8IWL2